WebHyperthermophilic archaea offer certain advantages as models of genome replication, and Sulfolobus Y-family polymerases Dpo4 (S. solfataricus) and Dbh (S. acidocaldarius) have been studied intensively in vitro as biochemical and structural models of trans-lesion DNA synthesis (TLS). However, the gen … WebThe resulting plasmid, called p1947, therefore expresses a chimeric polym- erase consisting of the thumb, finger, and palm domains of Dbh and the LF domain of the Dpo4 polymerase (Dbh-LF-Dpo4 ...
Lesion-Induced Mutation in the Hyperthermophilic Archaeon
WebDbh is a Y-family translesion DNA polymerase from Sulfolobus acidocaldarius, an archaeal species that grows in harsh environmental conditions. Biochemically, Dbh displays a distinctive mutational profile, creating single-base deletion mutations at extraordinarily high frequencies (up to 50 %) in specific repeat sequences. WebOct 5, 2001 · The authors suggest that Dbh may not operate by the type of induced-fit mechanism proposed for replicative polymerases such as the T7 enzyme, Taq polymerase, and mammalian polβ ( Kunkel and Bebenek 2000 ). This suggestion is supported by comparing the Dpo4 structure (with DNA) with that of Dbh (without DNA) ( Figure 1B ). ginny meets harry\u0027s parents fanfiction
1H, 13C, and 15N backbone resonance assignments of the
WebDNA Polymerase Catalysis in the Absence of Watson−Crick Hydrogen Bonds: Analysis by Single-Turnover Kinetics . × Close Log In. Log in with Facebook Log in with Google. or. Email. Password. Remember me on this computer. or reset password. Enter the email address you signed up with and we'll email you a reset link. ... WebNov 1, 2001 · A high error rate is observed for the Dbh polymerase in a range of 10 −2 –10 −3 for all 12 base substitution mispairs. The crystal structure of Dbh reveals an overall architecture resembling... WebDec 19, 2007 · Dbh is a Y family translesion DNA polymerase that accurately bypasses some damaged forms of deoxyguanosine, but also generates single-base deletion errors at frequencies of up to 50%, in specific hot spot sequences. We describe preinsertion binary, insertion ternary, and postinsertion binary crystal structures of Dbh synthesizing DNA … ginny medium logo stripe crossbody bag