Web22 jul. 2024 · HepaRG spheroids were used at 10 days after seeding and exposed for 24 and 48 hours to certain selected chemical compounds (methylmethansulfonate (MMS), … Web16 nov. 2024 · The HepaRG MN assay was 100% specific and 93% accurate in detecting genotoxic potential of the 28 compounds. Quantitative comparison of the MN concentration-response data using benchmark dose analysis showed that most of the …
Flow cytometric micronucleus assay and TGx-DDI transcriptomic …
Web4 feb. 2024 · Indeed, the flow cytometry-based micronucleus (MN) assay has been adapted for use with HepaRG™ cells [41, 42], and a variety of investigators have used … Web27 feb. 2024 · The in vitro micronucleus (MN) assay is a component of most test batteries used in assessing potential genotoxicity. Our previous study adapted metabolically … scotland bucket hat
CYP450 Activity in HepaRG Cells Download Table - ResearchGate
Web11 nov. 2024 · To fully differentiate into hepatocyte-like cells and reach optimal expression of metabolism enzymes, HepaRG cells are conventionally cultured for 14 days in the presence of DMSO after an initial 14-day period of proliferation to achieve high-density confluence (Aninat et al. 2006; Cerec et al. 2007 ). WebMicronucleus induction was seen in cultured meiotic mouse sperm cells at dose levels approaching toxicity, and a rat dominant lethal test was also positive, neither of which is too surprising since male germ cells are among the most sensitive cell targets. Positive results have been reported for strand breaks and DNA degradation in in vitro ... Web3 nov. 2011 · The micronucleus test was also performed after 7-day repeat exposure of HepaRG cells to the chemicals. Noticeably, a time-dependent effect was obtained with … scotland budget 2022-23