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Heparg micronucleus

Web22 jul. 2024 · HepaRG spheroids were used at 10 days after seeding and exposed for 24 and 48 hours to certain selected chemical compounds (methylmethansulfonate (MMS), … Web16 nov. 2024 · The HepaRG MN assay was 100% specific and 93% accurate in detecting genotoxic potential of the 28 compounds. Quantitative comparison of the MN concentration-response data using benchmark dose analysis showed that most of the …

Flow cytometric micronucleus assay and TGx-DDI transcriptomic …

Web4 feb. 2024 · Indeed, the flow cytometry-based micronucleus (MN) assay has been adapted for use with HepaRG™ cells [41, 42], and a variety of investigators have used … Web27 feb. 2024 · The in vitro micronucleus (MN) assay is a component of most test batteries used in assessing potential genotoxicity. Our previous study adapted metabolically … scotland bucket hat https://h2oattorney.com

CYP450 Activity in HepaRG Cells Download Table - ResearchGate

Web11 nov. 2024 · To fully differentiate into hepatocyte-like cells and reach optimal expression of metabolism enzymes, HepaRG cells are conventionally cultured for 14 days in the presence of DMSO after an initial 14-day period of proliferation to achieve high-density confluence (Aninat et al. 2006; Cerec et al. 2007 ). WebMicronucleus induction was seen in cultured meiotic mouse sperm cells at dose levels approaching toxicity, and a rat dominant lethal test was also positive, neither of which is too surprising since male germ cells are among the most sensitive cell targets. Positive results have been reported for strand breaks and DNA degradation in in vitro ... Web3 nov. 2011 · The micronucleus test was also performed after 7-day repeat exposure of HepaRG cells to the chemicals. Noticeably, a time-dependent effect was obtained with … scotland budget 2022-23

Benzo[a]pyrene (B[a]P) induced micronuclei in HepaRG cells using …

Category:Micronucleus Induction - an overview ScienceDirect Topics

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Heparg micronucleus

Adaptation of the in vitro micronucleus assay for genotoxicity …

Web27 sep. 2024 · The HepaRG MN assay was 100% specific and 93% accurate in detecting genotoxic potential of the 28 compounds. Quantitative comparison of the MN … Web3D hepatotoxicity assay using HepaRG spheroids; 3D brain microtissue combined with high-content-imaging end points; Mitochondrial Toxicity. Functional mitochondrial toxicity assay ... In vitro micronucleus test (HCS CHO-K1 cells) In vitro micronucleus test (Flow cytometry TK6 cells) pH3 and pH2AX genotoxicity assay;

Heparg micronucleus

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WebHepaRG™ cells are metabolically competent cells that express Phase I and II metabolic enzymes and differentiate into mature hepatocyte-like cells, making them ideal for toxicity testing.

WebStudy design of the micronucleus test in HepaRG cells. Differentiated HepaRG cells were prepared as previously described ( 15 ): after seeding at low density, they were allowed … WebThe results demonstrated that HepaRG spheroids showed generally higher sensitivity in detecting several indirect-acting genotoxicants (require metabolic activation) compared …

WebOur study investigated whether genotoxicity potency differed as a consequence of structural differences, assessing micronuclei in vitro in HepaRG cells which express metabolising … WebThe bone marrow micronucleus (MN) assay with rodents is at present the most widely used in vivo test. The majority of studies indicate that it detects only 5-6 out of 10 carcinogens while...

Web18 aug. 2024 · Human HepaRG™ cell cultures were exposed to increasing concentrations of 12 test chemicals in parallel 96-well plates (four test chemicals per plate) for …

Web28 jan. 2024 · Micronuclei are small, membrane-bound nuclear bodies containing DNA, which are separated from the main nucleus during mitosis and can therefore be readily … scotland budget deficitWeb3 nov. 2011 · HepaRG™ cells are metabolically competent cells that express Phase I and II metabolic enzymes and differentiate into mature hepatocyte-like cells, making them ideal … scotland budget deficit 2020Web12 apr. 2024 · The number of micronucleated BNCs among approximately 2,000 BNCs (approximately 667 cells/slide, triplicate tissues) was counted if possible, and the frequency of micronucleated BNCs (%MN) was calculated. Mitotic cells, multinucleated cells with four or more nuclei, and apoptotic cells were excluded from the MN analysis. scotland budget 23/24Web1 apr. 2024 · However, currently standardised in vitro methods for genotoxicity testing using mammalian cells (HPRT and TK assays, micronucleus assay) are tedious and not suitable for high-throughput testing. One of the promising markers of DNA damage response for genotoxicity assessment seems to be the phosphorylation of the histone variant H2AX [ 5 ]. scotland budgetWeb15 jan. 2014 · HepaRG cells, which have been shown to express various phase I and phase II enzymes, transporters, and nuclear factors, including the constitutively activated … scotland building regsWeb17 aug. 2015 · Moreover, for all these compounds, the concentrations inducing the γH2AX activation in HepaRG cells were compatible with data available in the literature with the … scotland budget 2023-24WebWe determine the performance of comet and micronucleus assays in HepaRG cells to predict in vivo genotoxins based on the list of compounds published by European … premature baby feeding schedule