Phosl lectin
WebPhoSL-hIgG1 is a lectin fused to human IgG constant region and specifically binds to α1-6 fucosylated N-glycans only and not other types of fucosylated oligosaccharides, such as α1-2-, α1-3-, and α1-4-fucosylated glycans. ... and afucosylated CHO cells stained with the PhoSL-hIgG1 (right). Anti-hIg PE was used as secondary Ab. Price is $200 ... WebMay 17, 2024 · The core α1–6 fucosylation-specific lectin from a mushroom Pholiota squarrosa (PhoSL) is a potential tool for precise diagnosis of cancers. This lectin consists of only 40 amino acids and can be chemically synthesized. We showed here that a synthesized PhoSL peptide formed a trimer by gel filtration and chemical cross-linking assays, and …
Phosl lectin
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WebJun 15, 2015 · PhoSL lectin, as well as PhoSL-antibody ELISA, may prove to be effective tools for this purpose. Furthermore, we evaluated the efficacy of our developed lectin–antibody ELISA kit in various conditions (Fig. 6, Fig. 7) like previous our study. Our findings indicated that serum dilution from 10 to 100-fold can be measured in PhoSL … WebThe lectin from a mushroom Pholiota squarrosa (PhoSL) shows a clear specificity to core fucosylation, without recognizing those with other types of fucosylation, such as the H type via the α1-2...
WebJul 31, 2014 · Taken together, PhoSL is the most suitable lectin for recognizing core fucose and might play a pivotal role in the future of fucosylation-biology. Figure & Legends Figure & Legends Fig. 1. Immunohistochemical staining of PhoSL in the original cancer tissue. Left panel: positive staining of AAL in the original colorectal cancer tissues. WebJul 6, 2024 · The lectin from a mushroom Pholiota squarrosa (PhoSL) shows a clear specificity to core fucosylation, without recognizing those with other types of …
Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 WebAug 25, 2024 · Here, we observed that the S proteins of the ancestral (Wuhan) and Omicron strains bind with Pholiota squarrosa lectin (PhoSL), a 40-amino-acid chemically synthesised peptide specific to core-fucosylated N-glycans. The affinities were at a low nanomolar level, which were ~ 1000-fold stronger than those between PhoSL and the core-fucosylated N ...
WebMay 17, 2024 · This lectin consists of only 40 amino acids and can be chemically synthesized. We showed here that a synthesized PhoSL peptide formed a trimer by gel …
WebMay 17, 2024 · Lectins are proteins with carbohydrate-binding ability. In plants, they have been obtained from leaves, weeds, roots, rhizomes, bulbs, pods, seeds, fruits and flowers. eagle one emichWebOct 30, 2024 · To investigate functional loss by FUT8 gene ablation, lectin binding was tested using a lectin from the mushroom Pholiota squarrosa (PhoSL), which shows a specific binding property for core ... eagle on dollar bill meaningWebOct 5, 2012 · The purified lectin was designated as PhoSL ( P. squarrosa lectin). SDS-PAGE, MALDI-TOF mass spectrometry, and N-terminal amino acid sequencing indicate that PhoSL has a molecular mass of 4.5 kDa … cs.landmarkworldwide.com course supervisorWebJan 30, 2016 · Fucosylation levels of AGP using AAL and PhoSL lectins. Fucosylation levels of AGP were analysed with an enzyme-linked lectin assay (ELLA) using A. aurantia lectin (AAL) which recognizes fucoses α1-2, α1-3 or α1-6-linked [7] and with P. squarrosa lectin (PhoSL) which recognizes only α1-6-linked fucose [24]. Both assays were optimized with ... eagle on cactus eating snakeWebTo complement the lectin profiling provided by the CFG array, we used AAL, LCA and PhoSL to perform lectin blots of protein extracts from eight CRC cell lines (HT29, Caco-2, SW480, SW620, DLD-1, HCT116, COLO205 adherent growth fraction and COLO205 suspension growth fraction). This set of CRC lines covers a broad range of CRC histopathological ... csl and kmWebThe BLAST search revealed that PhoSL has 85% sequence homology (22/26 amino acids) with the ␣ 1– 6-linked fucose- specific lectin from R. stolonifer . RSL has also been isolated as a core... csl alloy wheelsWebALC offers the broadest, most versatile line of lecithin-based products on the market. Under the world-famous ALCOLEC® trademark, American Lecithin markets a series of natural … csl analysis